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dcfh da fluorescent dye  (MedChemExpress)


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    MedChemExpress dcfh da fluorescent dye
    Dcfh Da Fluorescent Dye, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1053 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dcfh+da+fluorescent+dye/H2DCFDA/pm41616844-64-20-24
    Average 99 stars, based on 1053 article reviews
    dcfh da fluorescent dye - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Lactate promotes cuproptosis in acute kidney injury by activating H3K18 lactylation-dependent upregulation of HSPA6 expression.
    Article Snippet: .. Detection of ROS, GSH, SOD, Cu2+, lactate, and ATP levels For intracellular ROS detection, cells were incubated with 1 μM DCFH-DA fluorescent dye (HY-D0940, MCE, USA) for 15 min in the dark, and fluorescence signal was observed by flow detection. ..

    Fluorescence:

    Article Title: Lactate promotes cuproptosis in acute kidney injury by activating H3K18 lactylation-dependent upregulation of HSPA6 expression.
    Article Snippet: .. Detection of ROS, GSH, SOD, Cu2+, lactate, and ATP levels For intracellular ROS detection, cells were incubated with 1 μM DCFH-DA fluorescent dye (HY-D0940, MCE, USA) for 15 min in the dark, and fluorescence signal was observed by flow detection. ..



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    (a) The cytotoxicity of different concentrations <t>of</t> <t>PDA@MS-NH</t> 2 @HIF-1α nanomotors against normal HAECs under hypoxic conditions (NIR: 808 nm, 1.5 W/cm 2 ; n = 3, ∗ P < 0.05, ∗∗∗ P < 0.001). (b) Representative CLSM images and (c) flow cytometry analysis of intracellular <t>ROS</t> detection following treatment with various specimens for 12 h (Blank-NP: PDA@MS-NH 2 ; NP/pDNA: PDA@MS-NH 2 @HIF-1α; Green: ROS; Scale bar: 200 μm). (d) H 2 O 2 concentration of HAECs co-cultured with different samples ( n = 3, ∗∗∗ P < 0.001). (e) Confocal microscopic observation of gene transfection efficiency of PDA@MS-NH 2 @HIF-1α nanomotors in HAECs under hypoxic conditions. The eGFP was implemented as a control to ascertain the transfection efficiency, and the intensity of color directly corresponded to the level of fluorescence (Scale bars: 20 μm; Green: eGFP; Blue: DAPI). (f) The HIF-1α and (g) VEGF protein expression in culture supernatants of HAECs treated with different specimens ( n = 3, ∗∗∗ P < 0.001).
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    (a) The cytotoxicity of different concentrations <t>of</t> <t>PDA@MS-NH</t> 2 @HIF-1α nanomotors against normal HAECs under hypoxic conditions (NIR: 808 nm, 1.5 W/cm 2 ; n = 3, ∗ P < 0.05, ∗∗∗ P < 0.001). (b) Representative CLSM images and (c) flow cytometry analysis of intracellular <t>ROS</t> detection following treatment with various specimens for 12 h (Blank-NP: PDA@MS-NH 2 ; NP/pDNA: PDA@MS-NH 2 @HIF-1α; Green: ROS; Scale bar: 200 μm). (d) H 2 O 2 concentration of HAECs co-cultured with different samples ( n = 3, ∗∗∗ P < 0.001). (e) Confocal microscopic observation of gene transfection efficiency of PDA@MS-NH 2 @HIF-1α nanomotors in HAECs under hypoxic conditions. The eGFP was implemented as a control to ascertain the transfection efficiency, and the intensity of color directly corresponded to the level of fluorescence (Scale bars: 20 μm; Green: eGFP; Blue: DAPI). (f) The HIF-1α and (g) VEGF protein expression in culture supernatants of HAECs treated with different specimens ( n = 3, ∗∗∗ P < 0.001).
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    Image Search Results


    (a) The cytotoxicity of different concentrations of PDA@MS-NH 2 @HIF-1α nanomotors against normal HAECs under hypoxic conditions (NIR: 808 nm, 1.5 W/cm 2 ; n = 3, ∗ P < 0.05, ∗∗∗ P < 0.001). (b) Representative CLSM images and (c) flow cytometry analysis of intracellular ROS detection following treatment with various specimens for 12 h (Blank-NP: PDA@MS-NH 2 ; NP/pDNA: PDA@MS-NH 2 @HIF-1α; Green: ROS; Scale bar: 200 μm). (d) H 2 O 2 concentration of HAECs co-cultured with different samples ( n = 3, ∗∗∗ P < 0.001). (e) Confocal microscopic observation of gene transfection efficiency of PDA@MS-NH 2 @HIF-1α nanomotors in HAECs under hypoxic conditions. The eGFP was implemented as a control to ascertain the transfection efficiency, and the intensity of color directly corresponded to the level of fluorescence (Scale bars: 20 μm; Green: eGFP; Blue: DAPI). (f) The HIF-1α and (g) VEGF protein expression in culture supernatants of HAECs treated with different specimens ( n = 3, ∗∗∗ P < 0.001).

    Journal: Materials Today Bio

    Article Title: NIR light-propelled Janus Polydopamine@Mesoporous silica gene delivery nanomotor for the enhanced gene treatment of critical limb ischemia

    doi: 10.1016/j.mtbio.2025.101950

    Figure Lengend Snippet: (a) The cytotoxicity of different concentrations of PDA@MS-NH 2 @HIF-1α nanomotors against normal HAECs under hypoxic conditions (NIR: 808 nm, 1.5 W/cm 2 ; n = 3, ∗ P < 0.05, ∗∗∗ P < 0.001). (b) Representative CLSM images and (c) flow cytometry analysis of intracellular ROS detection following treatment with various specimens for 12 h (Blank-NP: PDA@MS-NH 2 ; NP/pDNA: PDA@MS-NH 2 @HIF-1α; Green: ROS; Scale bar: 200 μm). (d) H 2 O 2 concentration of HAECs co-cultured with different samples ( n = 3, ∗∗∗ P < 0.001). (e) Confocal microscopic observation of gene transfection efficiency of PDA@MS-NH 2 @HIF-1α nanomotors in HAECs under hypoxic conditions. The eGFP was implemented as a control to ascertain the transfection efficiency, and the intensity of color directly corresponded to the level of fluorescence (Scale bars: 20 μm; Green: eGFP; Blue: DAPI). (f) The HIF-1α and (g) VEGF protein expression in culture supernatants of HAECs treated with different specimens ( n = 3, ∗∗∗ P < 0.001).

    Article Snippet: In order to assess the ability of PDA@MS-NH 2 nanomotors to eliminate intracellular ROS, the production of intracellular ROS induced by lipopolysaccharide (LPS) in Human amniotic epithelial cells (HAECs) was quantified using the ROS-sensitive fluorescent dye 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA; Beyotime Biotechnology, China).

    Techniques: Flow Cytometry, Concentration Assay, Cell Culture, Transfection, Control, Fluorescence, Expressing